Salmeterol, a Long‐Acting &bgr;2‐Adrenergic Receptor Agonist, Inhibits Macrophage Activation by Lipopolysaccharide From Porphyromonas gingivalis

doi:10.1902/jop.2017.160464

Paper

Salmeterol, a Long‐Acting &bgr;2‐Adrenergic Receptor Agonist, Inhibits Macrophage Activation by Lipopolysaccharide From Porphyromonas gingivalis

Published Jul 1, 2017 · Monika Sharma, L. Patterson, E. Chapman

Journal of Periodontology

Q1 SJR score

21

Citations

0

Influential Citations

Full textSemantic Scholar

Abstract

BACKGROUND Salmeterol is a long-acting β2-adrenergic receptor agonist used to treat chronic obstructive pulmonary disease. The authors of the current study previously showed that preincubation of primary microglial-enriched cells with salmeterol could inhibit the inflammatory response induced by Escherichia coli lipopolysaccharide (LPS), a Toll-like receptor (TLR)-4 agonist. In this study, the authors sought to determine if salmeterol had a similar inhibitory effect on the inflammatory response of the murine macrophage cell line RAW264.7 and human monocyte THP-1 to LPS from Porphyromonas gingivalis (PgLPS), an oral microbe implicated in the pathogenesis of periodontal disease. METHODS RAW264.7 and THP-1 cells were pretreated with salmeterol, followed by PgLPS, and monitored for production of inflammatory mediators by enzyme-linked immunosorbent assay. The nitric oxide concentration and nuclear factor-kappa B (NF-κB) activity were measured by Griess method and secretory alkaline phosphatase reporter activity assay, respectively. Reverse-transcriptase polymerase chain reaction and immunoblot analysis were used to measure messenger RNA and protein levels. Nuclear translocation of NF-κB was detected by immunofluorescence. RESULTS Pretreatment with salmeterol significantly inhibited production of proinflammatory mediators by RAW264.7 and THP-1 cells. Salmeterol downregulated PgLPS-mediated phosphorylation of the extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase but not p38 mitogen-activated protein kinases (MAPKs). Salmeterol also attenuated activation of NF-κB via inhibition of nuclear translocation of p65-NFκB, the transcriptional activity of NF-κB and IκBα phosphorylation. CONCLUSION Salmeterol can significantly inhibit activation of macrophage-mediated inflammation by PgLPS, suggesting that use of salmeterol may be an effective treatment in inhibiting or lessening the inflammatory response mediated through TLR pathway activation.

In Vitro Study

Study Snapshot

Salmeterol effectively inhibits macrophage-mediated inflammation by Porphyromonas gingivalis lipopolysaccharide, suggesting it may be an effective treatment for periodontal disease.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

Salmeterol, a Long‐Acting &bgr;2‐Adrenergic Receptor Agonist, Inhibits Macrophage Activation by Lipopolysaccharide From Porphyromonas gingivalis

Sign up to use Study Snapshot

References

Macrophage activating factor: A potential biomarker of periodontal health status.

Salivary MAF levels positively correlate with tissue destruction in periodontal diseases, making it a potential valuable biomarker for assessing periodontal health status.

Multiple Apical Periodontitis Influences Serum Levels of Cytokines and Nitric Oxide.

Multiple apical periodontitis in rats alters serum levels of inflammatory cytokines and nitric oxide, affecting blood homeostasis.

ROLE OF INNATE IMMUNITY FACTORS IN PERIODONTITIS PATHOGENESIS

Innate immunity factors play a crucial role in periodontitis pathogenesis, and understanding their role may help develop effective therapies for this disease.

Association between periodontal disease and pregnancy outcomes.

Periodontal disease is a probable risk for adverse pregnancy outcomes like preterm delivery and low birth weight, highlighting the need for health workers to promote good oral health among women.

Molecular aspects of the pathogenesis of periodontitis.

Periodontitis pathogenesis involves complex interactions between microbial and host factors, with inflammation and dysbiosis affecting tissue regeneration and disease progression.

Citations

Visualize the time dynamics and research trends of macrophage associated periodontitis research from 2004 to 2023: Bibliometrix analysis

Macrophage associated periodontitis research has shown significant progress from 2004 to 2023, with China being the most productive country and Sichuan University being the most productive institution.

Chronic stress influences the macrophage M1-M2 polarization balance through β-adrenergic signaling in hepatoma mice.

Chronic stress disrupts the M1-M2 polarization balance of tumor-associated macrophages through -adrenergic signaling, promoting liver cancer growth.

The Link between Periodontal Disease and Asthma: How Do These Two Diseases Affect Each Other?

Poor oral hygiene and asthma may have a link, with medications, hypoxia, and breathing behavior potentially triggering periodontal disease, while oral bacteria may contribute to asthma onset and exacerbation.

NAT10 regulates the LPS-induced inflammatory response via the NOX2-ROS-NF-κB pathway in macrophages.

NAT10 accelerates LPS-induced inflammation in macrophages, and its inhibitor Remodelin shows potential therapeutic significance in periodontitis treatment by reducing macrophage infiltration and bone resorption.

Adrenergic receptor signaling regulates the CD40-receptor mediated anti-tumor immunity

Stress-induced 2AR signaling reduces the efficacy of anti-CD40 antibodies in cold tumors, offering a new combinatorial approach to improve clinical outcomes in patients.

The β‐adrenergic hypothesis of synaptic and microglial impairment in Alzheimer's disease

2AR agonists may provide therapeutic value in combination with novel treatments for Alzheimer's disease by improving learning and memory, and preventing synaptic dysfunction and neuroinflammation.

High concentrations of Porphyromonas gingivalis-LPS downregulate Tlr4 and modulate phosphorylation of ERK and AKT in murine cementoblasts.

High concentrations of Porphyromonas gingivalis-LPS reduce cell viability and downregulate Tlr4, affecting inflammatory responses and periodontal tissue destruction.

Effects of a β2-adrenergic receptor blocker on experimental periodontitis in spontaneously hypertensive rats.

Butoxamine effectively prevents periodontitis by maintaining alveolar bone mass and attachment level by suppressing osteoclast activity in spontaneously hypertensive rats.

Adrenergic signalling in osteoarthritis.

Targeting adrenergic signaling in osteoarthritis may offer potential for prevention and treatment, as it contributes to the pathogenesis of the joint disorder.