B. Fransson, U. Ragnarsson
Dec 4, 1998
Citations
0
Influential Citations
43
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The application of the chiral reagent (+)-1-(9-fluorenyl)ethyl chloroformate, also known as (+)-FLEC and originally introduced for the separation of enantiomers of α-amino acids and the determination of their optical purity by HPLC, has been extended to enantioseparation within a set of α-hydroxy acids. Retention of the acidic diastereomeric carbonate derivatives thereby formed is conveniently regulated by the pH of the mobile phase and separation can generally be accomplished within about 30 min under isocratic conditions with tetrahydrofuran as organic modifier. Of six α-hydroxy acids so far examined five had separation factors of 1.24 or higher, whereas for malic acid it was in the range 1.10–1.15.