W. Allen
1950
Citations
0
Influential Citations
369
Citations
Quality indicators
Journal
The Journal of Clinical Endocrinology and Metabolism
Abstract
THERE are many color reactions which have been found useful in the quantitative determination of several of the steroids. The first of these, employing phenolsulfonic acid, was discovered by Kober in 1931 (1) and has been employed in various modifications for the determination of the urinary estrogens. The second, utilizing m-dimtrobenzene, was discovered by Zimmermann in 1936 and has become very useful for the determination of the urinary 17-ketosteroids (2). Both of these reactions give characteristic colors with specific absorption maxima when pure compounds are used. However, in urinary extracts there are chromogenic impurities which make a correction necessary unless the compounds being measured are present in considerable amount. These two color reactions, and modifications thereof, have actually almost completely replaced bioassay as a method for studying the excretion of the steroids. Also they are in many instances more valuable than bio-assay since they make possible the study of relatively inac...