L. Otvos, L. Ürge, M. Hollósi
1990
Citations
0
Influential Citations
51
Citations
Journal
Tetrahedron Letters
Abstract
Abstract A new reagent for custom synthesis of N-glycosylated peptides, Fmoc-Asn[GlcNAc(1»4)-GlcNAc]-OH, has been prepared. The O-glycosidic bond survived the trifluoroacetic acid cleavage. High coupling efficiencies were obtained when this glycoamino acid derivative was used for solid-phase synthesis of a test peptide and a T cell epitopic dodecapeptide containing an Asn residue in mid-chain position. An automated peptide synthesizer was used to produce the parent Asn peptide and its N-acetyl-glycosyl mono- and disaccharide derivatives.