L. Ge
2012
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0
Influential Citations
1
Citations
Journal
Chemical Reagents
Abstract
The mechanism interaction between four metalloporphyrin complexes including 5-(4-carboxyphenyl)-10,15,20-triphenylporphyrin zinc,cobalt,nickel,manganese(MCPPZn,MCPPCo,MCPPNi,MCPPMnCl) and bovine serum albumin(BSA) was studied by means of fluorescent spectroscopy.The rnechanism of fluorescence quenching of BSA caused by four metalloporphyrin complexes was investigated.The binding constants were measured at different temperatures,which showed that the combinations between these four metalloporphyrin complexes and BSA were large,and the sort of quenching on BSA was a static quenching procedure.According to the thermodynamic parameters,the main sorts of binding force could be confirmed as electrostatic force between MCPPZn and BSA,hydrogen bond and van der Waals force between MCPPCo(25 or 42 ℃),MCPPNi,MCPPMnCl and BSA.The differences of binding constants and the sorts of quenching force were produced mainly by electro-negativity and electron number on outer d-orbit of central metal ion.