Stimulation of collagen synthesis by the anabolic steroid stanozolol.

doi:10.1046/J.1523-1747.1998.00431.X

Paper

Stimulation of collagen synthesis by the anabolic steroid stanozolol.

Published Dec 1, 1998 · V. Falanga, A. Greenberg, L. Zhou

The Journal of investigative dermatology

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61

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1

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Abstract

There is evidence that anabolic steroids, which are derived from testosterone and have markedly less androgenic activity, promote tissue growth and enhance tissue repair; however, the mechanisms involved in their anabolic activities remain unclear. In this report, we measured the effect of the anabolic steroid stanozolol on cell replication and collagen synthesis in cultures of adult human dermal fibroblasts. Stanozolol (0.625-5 microg per ml) had no effect on fibroblast replication and cell viability (p = 0.764) but enhanced collagen synthesis (p < 0.01) in a dose-dependent manner (r = 0.907). Stanozolol also increased (by 2-fold) the mRNA levels of alpha1 (I) and alpha1 (III) procollagen and, to a similar extent, upregulated transforming growth factor-beta1 (TGF-beta1) mRNA and peptide levels (p < 0.001). There was no stimulation of collagen synthesis by testosterone. The stimulatory effects of stanozolol on collagen synthesis were blocked by a TGF-beta1 anti-sense oligonucleotide, by antibodies to TGF-beta, and in dermal fibroblast cultures derived from TGF-beta1 knockout mice. We conclude that collagen synthesis is increased by the anabolic steroid stanozolol and that, for the most part, this effect is due to TGF-beta1. These findings point to a novel mechanism of action of anabolic steroids.

In Vitro Study

Study Snapshot

Stanozolol stimulates collagen synthesis in human dermal fibroblasts, mainly due to TGF-beta1, highlighting a novel mechanism of action for anabolic steroids.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Stimulation of collagen synthesis by the anabolic steroid stanozolol.

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References

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Venous ulcer fibroblasts are unresponsive to TGF-, potentially causing faulty extracellular matrix deposition needed for reepithelialization and wound healing.

TGF-β3 stimulates and regulates collagen synthesis through TGF-β1- dependent and independent mechanisms

TGF-3 stimulates collagen synthesis through both TGF-1-dependent and independent pathways, providing fine-tuning to the overall TGF- induced collagen deposition program.

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Peak E, a major constituent of contaminated L-tryptophan, stimulates fibroblast activation and collagen synthesis, potentially contributing to the development of fibrosis in eosinophilia myalgia syndrome.

Stanozolol as a novel therapeutic agent in dermatology.

Stanozolol is a safe and effective treatment for various dermatological conditions, including hereditary angioedema, urticaria, Raynaud's phenomenon, and cryofibrinogenemia and lipodermatosclerosis.

Inhibition of TGF-beta 3 (but not TGF-beta 1 or TGF-beta 2) activity prevents normal mouse embryonic palate fusion.

TGF-beta 3 plays a specific, isoform-specific role in mouse embryonic palate fusion, while TGF-beta 1 or TGF-beta 2 do not have a significant effect on palate development.

Citations

A Pilot Study of the Clinical Effectiveness of a Single Intra-Articular Injection of Stanozolol in Canines with Knee Degenerative Joint Disease and Its Correlation with Serum Interleukin-1β Levels

A single intra-articular stanozolol injection in canine knees with degenerative joint disease (DJD) significantly reduced pain intensity, suggesting potential in managing this condition in dogs.

Impacts of Chronic Mesterolone Intake on Postmortem Changes in Heart of Adult Male Albion Rats (Biochemical and Histopathological Study)

Chronic Mesterolone intake significantly alters cardiac muscle energy content, oxidative status, and histological architecture in the early postmortem period.

Anabolic Steroids in Fattening Food-Producing Animals—A Review

Anabolic steroids in animal fattening cause significant tissue damage and public health concerns, and new detection methods are needed to ensure meat safety.

THE EFFECTS OF STANOZOLOL AND HIGH PROTEIN DIET ON COLON ANASTOMOSIS

A high protein diet and stanozolol combined can improve anastomotic healing after colorectal surgery in rats, but further research is needed.

Increased expression of aquaporin-1 in dermal fibroblasts and dermal microvascular endothelial cells possibly contributes to skin fibrosis and edema in patients with systemic sclerosis.

AQP1 overexpression in skin cells may contribute to inflammation, vasculopathy, and tissue fibrosis in systemic sclerosis patients by regulating tissue edema and cell migration.