Stolonidiol: Synthesis, Target Identification, and Mechanism for Choline Acetyltransferase Activation.

doi:10.1021/jacs.7b01083

Paper

Stolonidiol: Synthesis, Target Identification, and Mechanism for Choline Acetyltransferase Activation.

Published Apr 17, 2017 · J. W. Mason, Cullen L. Schmid, L. Bohn

Journal of the American Chemical Society

Q1 SJR score

14

Citations

0

Influential Citations

Full textSemantic Scholar

Abstract

Stolonidiol, a marine natural product, has been reported to potentiate the activity of choline acetyltransferase (ChAT), the enzyme that produces the neurotransmitter acetylcholine. Here we report the total synthesis of stolonidiol starting from (R)-(+)-limonene. To identify the mechanism by which ChAT activity is increased, we sought to identify the biological target of stolonidiol. We show that stolonidiol binds to the phorbol ester binding site of protein kinase C (PKC), induces translocation of PKC to the cell membrane, and activates kinase activity. Furthermore, we confirmed the increase in ChAT activity observed upon treatment of cells with stolonidiol and show that this effect is mediated by PKC. Collectively, our data strongly suggest that PKC activation by stolonidiol is responsible for the resulting potentiation of ChAT activity.

In Vitro Study

Study Snapshot

Stolonidiol increases choline acetyltransferase activity by binding to protein kinase C and activating it, leading to increased neurotransmitter acetylcholine production.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

Stolonidiol: Synthesis, Target Identification, and Mechanism for Choline Acetyltransferase Activation.

Sign up to use Study Snapshot

References

Citations

Phosphonium Ion-Tethered Secondary Amines for Chemospecific 5-Enolexo Aldol Condensations of 6-Ketoaldehydes.

This study presents a novel, highly selective 5-enolexo-exo-trig aldol condensation method for 6-ketoaldehydes, enabling the synthesis of diverse carboxaldehydes.

Marine Pharmacology in 2016–2017: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

In 2016-2017, preclinical marine pharmacology research identified 313 novel compounds with potential therapeutic applications, including antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral properties.

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

Fluorescent probes can aid in early diagnosis of neurodegenerative diseases by sensing and imaging biomarkers, offering potential for future improvements in this field.

Sangiangols A and B, Two New Dolabellanes from an Indonesian Marine Soft Coral, Anthelia sp.

Sangiangols A and B, two new dolabellanes from Anthelia sp. coral, show moderate cytotoxicity against an NBT-T2 cell line, suggesting a possible biosynthetic pathway.

Egg yolk phospholipids reverse scopolamine–induced spatial memory deficits in mice by attenuating cholinergic damage

Egg yolk phospholipids improve spatial memory deficits in mice by preventing cholinergic damage and regulating cholinergic biomarkers.

Ubiquitin-Proteasome Modulating Dolabellanes and Secosteroids from Soft Coral Clavularia flava

Six compounds from soft coral Clavularia flava show less cytotoxicity than known proteasome inhibitors, bortezomib and MG132, and show potential as a new proteasome inhibitor.

Advance in food-derived phospholipids: Sources, molecular species and structure as well as their biological activities

Food-derived phospholipids from various sources have diverse molecular species and structures, with aquatic-source phospholipids containing significant amounts of n-3 polyunsaturated fatty acids, which contribute to antioxidant activity, memory, immunity, and cardiovascular disease prevention.

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe

This study developed a ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening, using controlled perylene probe assembly and monomer-excimer transition.