Artificial Sweetener Enhances the Spread of Antibiotic Resistance Genes During Anaerobic Digestion.

doi:10.1021/acs.est.2c08673

Paper

Artificial Sweetener Enhances the Spread of Antibiotic Resistance Genes During Anaerobic Digestion.

Published Jul 20, 2023 · Guang Yang, Jinman Cao, Huiling Cui

Environmental science & technology

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27

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0

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Abstract

Artificial sweeteners have been frequently detected in the feedstocks of anaerobic digestion. As these sweeteners can lead to the shift of anaerobic microbiota in the gut similar to that caused by antibiotics, we hypothesize that they may have an antibiotic-like impact on antibiotic resistance genes (ARGs) in anaerobic digestion. However, current understanding on this topic is scarce. This investigation aimed to examine the potential impact of acesulfame, a typical artificial sweetener, on ARGs in anaerobic digestion by using metagenomics sequencing and qPCR. It was found that acesulfame increased the number of detected ARG classes and the abundance of ARGs during anaerobic digestion. The abundance of typical mobile genetic elements (MGEs) and the number of potential hosts of ARGs also increased under acesulfame exposure, suggesting the enhanced potential of horizontal gene transfer of ARGs, which was further confirmed by the correlation analysis between absolute abundances of the targeted ARGs and MGEs. The increased horizontal dissemination of ARGs may be associated with the SOS response induced by the increased ROS production, and the increased cellular membrane permeability. These findings indicate that artificial sweeteners may accelerate ARG spread through digestate disposal, thus corresponding strategies should be considered to prevent potential risks in practice.

Study Snapshot

Artificial sweeteners, such as acesulfame, can accelerate the spread of antibiotic resistance genes during anaerobic digestion, highlighting the need for strategies to prevent potential risks.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Artificial Sweetener Enhances the Spread of Antibiotic Resistance Genes During Anaerobic Digestion.

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References

Non-caloric artificial sweeteners modulate conjugative transfer of multi-drug resistance plasmid in the gut microbiota

Non-caloric artificial sweeteners can increase oxidative stress and boost the transfer of antibiotic resistance genes among gut microbiota and human pathogens.

Deciphering the role of microplastic size on anaerobic sludge digestion: Changes of dissolved organic matter, leaching compounds and microbial community.

The size of microplastics significantly impacts anaerobic sludge digestion, with larger particles promoting higher methane production and a more diverse microbial community.

Investigating changes in the characteristics of antibiotic resistance genes at different reaction stages of high solid anaerobic digestion with pig manure.

Rapid starting methods in high solid anaerobic digestion can accelerate start-up and increase biogas production by 312.5%, while reducing antibiotic resistance genes at the acidification stage.

Increasing the removal efficiency of antibiotic resistance through anaerobic digestion with free nitrous acid pretreatment.

Anaerobic digestion with free nitrous acid pretreatment effectively reduces antibiotic resistance genes in swine manure, improving biogas production and facilitating swine manure management.

Effects of heavy metals and antibiotics on performances and mechanisms of anaerobic digestion.

Anaerobic digestion efficiency can be limited by metals and antibiotics in organic wastes, and understanding their interaction mechanisms can enhance its performance and control potential issues.

Contributions of MOF-808 to methane production from anaerobic digestion of waste activated sludge.

MOF-808 significantly enhances methane production and biogas proportion in waste activated sludge by improving hydrolysis and syntrophic methanogenesis during anaerobic digestion.

Persulfate-based strategy for promoted acesulfame removal during sludge anaerobic fermentation: Combined chemical and biological effects.

Persulfate-based treatments effectively promote acesulfame removal during anaerobic fermentation in waste activated sludge, providing a novel and effective strategy for refractory pollutants removal.

Polyethylene terephthalate microplastic fibers increase the release of extracellular antibiotic resistance genes during sewage sludge anaerobic digestion.

Microplastic fibers in sewage sludge increase the release of extracellular antibiotic resistance genes, potentially accelerating their transport in the environment during treatment and disposal.

Non-caloric artificial sweeteners exhibit antimicrobial activity against bacteria and promote bacterial evolution of antibiotic tolerance.

Non-caloric artificial sweeteners exhibit antimicrobial activity against bacteria and promote bacterial evolution of antibiotic tolerance, highlighting the need for re-evaluation of risks due to their intensive usage.

Citations