The synthesis of 4-methylumbelliferyl alpha-ketoside of N-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase.

doi:10.1016/0003-2697(80)90056-1

Paper

DOI: 10.1016/0003-2697(80)90056-1

The synthesis of 4-methylumbelliferyl alpha-ketoside of N-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase.

Published 1980 · R. Myers, R. Lee, Y. Lee

Analytical biochemistry

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146

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Abstract

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Study Snapshot

4-methylumbelliferyl alpha-ketoside of N-acetylneuraminic acid is a suitable substrate for neuraminidase, with optimal pH values varying among different neuraminidases.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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References

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Characterization of neuraminidase activity of cultured human fibroblasts.

Cultured human fibroblasts have a maximum neuraminidase activity at pH 4.2-4.6, with a Km value of 3.6 10 3 M, and can be inactivated by sonication, freeze-thaw treatment, and storage at 70°C

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A rapid and sensitive assay for neuraminidase: Application to cultured flbroblasts

This study developed a rapid and sensitive assay for neuraminidase activity in cultured human skin fibroblasts, with the 2 3 isomer hydrolyzed twice as fast as the 2 6 isomer in genetic disorders.

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Analytical Biochemistry

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Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.

The sodium substrate-based fluorometric assay accurately detects neuraminidase activity in Vibrio cholerae, cultured fibroblasts, and human leucocytes, with reduced activity in mucolipidosis patients.

1979·798citations·M. Potier et al.·Analytical biochemistry

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Km values of influenza virus neuraminidases for a new fluorogenic substrate, 4-methylumbelliferone N-acetyl neuraminic acid ketoside.

The study synthesized a new fluorogenic substrate, MUN, and determined Km values for various neuraminidases, aiding in understanding influenza virus enzyme activity.

1978·32citations·J. Thomas et al.·Analytical biochemistry

Analytical biochemistry

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Synthesis of 4-methylumbelliferyl 1,2-cis-glycosides☆

4-methylumbelliferyl 1,2-cis-glycosides can be synthesized by condensing 4-methylumbelliferone with 1,2,3,4,6-penta-O-acetyl--d-glucopyranose and 1,2,3,4,6-penta-

1978·23citations·Marie-Christine Courtin-Duchateau et al.·Carbohydrate Research

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Recent sialidase substrates and methods are essential for evaluating enzyme activity, specificity, and screening inhibitors, aiding in understanding biological significance and developing novel treatments and diagnostics.

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The chemoenzymatic synthesis of the rare bacterial sugar pseudaminic acid, and its utilisation in the study of a potential pseudaminidase

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