Chen Li-gong
2006
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Journal
Chemical Industry and Engineering
Abstract
Fluorescein isothiocyanate(FITC) is an important fluorescence probe.In this paper 2′,7′-dichloride-5(6)-fluorescein isothiocyanate was obtained from 4-nitrophthalic acid,4-chloride-resoreinol and zinc chloride through condensation,reduction and isothiocyanatation in a total yield of 34.78%.The structure of the products were characterized by()~1H NMR,()~(13)C NMR,IR and MS,and the fluorescent properties of the products were determined.The results showed the fluorescent properties of the products were prior to that of FITC.