I. A. Natchev
Jun 1, 1988
Citations
0
Influential Citations
7
Citations
Journal
Phosphorus Sulfur and Silicon and The Related Elements
Abstract
Abstract Three-component condensation of glycine ethyl ester hydrochloride, formalin and hydrophosphorous compounds 1–3 was carried out to the esters 4–6, which after acid hydrolysis led to the free N-phosphino-, phosphonomethylated glycines 7–9. Strict selectivity in enzyme-catalyzed hydrolysis of phosphino-, phosphono acid esters was established. The enzyme phosphodiesterase I catalyzed the hydrolysis of the ester 4, 5 and 6 to the corresponding 10, 11 and 12. Lyophilyzed bee venom hydrolyzed the ethoxycarbonyl groups of 4–6 to the corresponding free glycines 7–9, and the enzyme alkaline phosphatase contributed to the hydrolysis only of the one from the two diethoxyphosphono groups of the substrate 6 to the monophosphono ester–13. Proteolytic enzymes α-chymotrypsin, protease and alkaline mesintericopeptidase catalyzed only hydrolysis of the ethoxycarbonyl groups of the substrates 5 and 6 to the free carboxylic acids 14 and 15 which after treatment with phosphodiesterase I or bee venom liberated glycines...