I. Kovács, Z. Kovács
Nov 1, 1991
Citations
0
Influential Citations
3
Citations
Journal
Journal of Labelled Compounds and Radiopharmaceuticals
Abstract
Ethyl methylmalonyl thioglycolate is a potential substrate analogue of methylmalonyl-coenzyme-A (methylmalonyl-CoA) in the investigation of propionic acid metabolism. To prove this hypothesis, the tracer ethyl [14CH3]methylmalonyl thioglycolate was synthesized via methyl-Meidrum's acid to carry out the biochemical examinations. The method described can also be used to synthesize [14CH3]methylmalonyl-CoA by transesterification of active labelled methylmalonyl thiophenyl ester. This latter intermediate is chemically stable when stored at room temperature, and the unstable [14CH3]methylmalonyl-CoA can be prepared in one step just preceeding the biochemical experiments.