Hiroaki Yamamoto, A. Matsuyama, Yoshinori Kobayashi
Jan 1, 2002
Citations
2
Influential Citations
57
Citations
Journal
Bioscience, Biotechnology, and Biochemistry
Abstract
The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate ((R)-ECHB) from ethyl 4-chloroacetoacetate was studied using whole recombinant cells of Escherichia coli expressing a secondary alcohol dehydrogenase of Candida parapsilosis. Using 2-propanol as an energy source to regenerate NADH, the yield of (R)-ECHB reached 36.6 g/l (more than 99% ee, 95.2% conversion yield) without addition of NADH to the reaction mixture.