Minoti Sharma, J. Alderfer, H. Box
Oct 1, 1983
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0
Influential Citations
4
Citations
Journal
Journal of Labelled Compounds and Radiopharmaceuticals
Abstract
Morpholinium [13C]formate is obtained by neutralizing an aqueous solution of equivalent amounts of sodium [13C]formate and hydrochloric acid with morpholine. Morpholinium [13C]formate is isolated in crystalline form and characterized. Treatment of 6-hydroxy-2,4,5-triaminopyrimidine sulfate with morpholinium [13C]formate at 200° affords [8-13C]guanine in 80% yield. There is no necessity to synthesize and isolate [13C]-N-formylmorpholine and the intermediate N5-[13C]formyl pyrimidine derivative. Mass spectrometric analysis shows that the molar isotopic concentration of 13C label in guanine is identical to that of the morpholinium [13C]formate and there is no isotopic scrambling. The same procedure when used with 4,5,6-triaminopyrimidine sulfate affords adenine in 85% yield.