Toyokazu Yoshida, M. Kito, M. Tsujii
Aug 1, 2001
Citations
0
Influential Citations
26
Citations
Journal
Biotechnology Letters
Abstract
Microbial oxidation of 2-[4-(3-methoxypropoxy)-3-methylpyridin-2-yl]methylthiobenzimidazole to a useful proton pump inhibitor, sodium 2-[[4-(3-methoxypropoxy)-3-methylpyridin-2-yl] methylsulfinyl]-1H benzimidazole (Rabeprazole), was examined in over 650 microorganisms. Rabeprazole-forming activity was distributed in molds. The mold with the highest activity was identified as Cunninghamella echinulata. Glucose, when added to the reaction mixture, gave the highest accumulation of Rabeprazole (6.9 mM, 2.5 g l−1) with a molar conversion ratio of 92% without the formation of the sulfone form as undesired product and resulted in the exclusive formation of (S) enantiomer (>99% e.e.).