M. Muralisankar, J. Haribabu, N. Bhuvanesh
Aug 1, 2016
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Influential Citations
58
Citations
Journal
Inorganica Chimica Acta
Abstract
Abstract N-ethyl-2-(phenyl(pyridin-2-yl)methylene)hydrazinecarbothioamide (HBpyeTsc) was obtained from 4-ethyl-3-thiosemicarbazide and 2-benzoylpyridine. The complexes (CuBpyeTscCl) and Ni[BpyeTsc]2Cl2 were synthesized from HBpyeTsc and [CuCl2·2H2O]/[NiCl2·6H2O]. The synthesized ligand and complexes were characterized by analytical and various spectroscopic techniques. The molecular structure of the ligand and complexes was determined by single crystal X-ray diffraction method. The single crystal X-ray diffraction revealed that CuBpyeTscCl and Ni[BpyeTsc]2Cl2 exhibited square planar and distorted octahedral geometry respectively. All of the synthesized compounds HBpyeTsc, CuBpyeTscCl and Ni[BpyeTsc]2Cl2 were studied for their interaction with calf thymus DNA (CT DNA) and bovine serum albumin (BSA). A DNA cleavage study showed that the complexes cleaved DNA without any external agent. The alterations in the secondary structure of the protein by the ligand and complexes were confirmed by synchronous fluorescence spectroscopic studies. Spectral evidences also showed good binding property of the complexes with the protein. An in vitro cytotoxicity study of the complexes found significant activity against lung cancer cell lines (A549) and less toxicity toward the normal cell lines (L929). The best results for the copper(II) and nickel(II) complexes are the IC50 values of 56.07 and 80.10 μM respectively.