Pan Hui
2006
Citations
0
Influential Citations
3
Citations
Journal
Chinese Journal of Applied Chemistry
Abstract
The synthesis of aminofluorescein,an important fluorescein probe,was studied systematically.A nitrofluorescein compound was obtained from 4-nitrophthalic acid and resoreinol in the presence of zinc(chloride) by melting reaction with a yield of 85.0%.Reduction of the nitrofluorescein compound with Na_(2)S/NaHS,Na_(2)S_(2),SnCl_(2),or H_(2)/Raney nickel yielded aminofluorescein compounds.5-Aminofluorescein and(6-aminofluorescein) were isolated by recrystallization of the obtained mixture in 6% hydrochloric acid with 39.6% and 14.5% yields respectively.The fluorescence intensity of the product,λ_(EX) and λ_(EM),were determined with a fluorometer.The purities of the products were identified with HPLC,and the structures of the products were charac-(terized) by ~(1)H NMR,~(13)C NMR,IR and MS.