E. Delikatny, S. Roman, R. Hancock
Jul 3, 1996
Citations
1
Influential Citations
41
Citations
Quality indicators
Journal
International Journal of Cancer
Abstract
The effect of the cationic lipophilic phosphonium salt tetraphenylphosphonium chloride (TPP) on a human malignant breast cell line, DU4475, was monitored with proton nuclear magnetic resonance (1H MRS). TPP caused a dose‐ and time‐dependent increase in resonances arising from MR‐visible lipid as measured by the CH2/CH3 ratio in the I‐dimensional 1H MR spectrum. Two‐dimensional MRS identified increases in the glycerophosphocholine/lysine cross‐peak ratio and corresponding decreases in the phosphocholine/lysine ratio in a dose‐dependent fashion in TPP‐treated cells. Lipid metabolic changes are discussed in the light of other MR experiments, and the data indicate that accumulation of MR‐visible lipids may arise from the rearrangement of phospholipids accompanying mitochondrial destruction or from the catabolism of phospholipids associated with early events in the cytotoxic process. © 1996 Wiley‐Liss, Inc.