L. Hayes, F. Plapp, L. Tilzer
Jul 8, 1975
Citations
0
Influential Citations
3
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Summary A single injection of the amino acid analog L-histidinol dihydrochloride reduced [ 3 H]-leucine incorporation into protein in vivo and partially disaggregated mouse liver polysomes into monosomes within 30 minutes. Isolated monosomes dissociated into 40S and 60S subunits during centrifugation in linear sucrose gradients containing 0.3 M KC1 and had a 4S to 5S molar RNA ratio of 0.7, indicating 0.7 tRNA and/or aminoacyl tRNA molecule per ribosome. No peptidyl tRNA was present. These results suggest that a single injection of histidinol rapidly produces monosomes which resemble runoff ribosomes.