W. Chao, M. Kai, J. Ishida
1988
Citations
0
Influential Citations
8
Citations
Journal
Analytica Chimica Acta
Abstract
Abstract 1,2-Diamino-4,5-ethylenedioxybenzene is shown to be a highly sensitive reagent for aromatic aldehydes, especially for benzaldehydes having a hydroxy group. The reagent reacts selectively with aromatic aldehydes at pH 3.0 (phosphate buffer) within 30 min at 60°C; the products fluoresce most intensely at pH 11. In the manual method, the lower limits of detection vary from 6 pmol ml−1 to 7 nmol ml−2. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase high-performance liquid chromatography. The fluorescent product from 4-hydroxybenzaldehyde is shown to be 2-(4-hydroxyphenyl)-5,6-ethylenedioxybenzimidazole.