S. Hara, M. Yamaguchi, Y. Takemori
1988
Citations
2
Influential Citations
30
Citations
Journal
Analytica Chimica Acta
Abstract
Abstract The reactivity of methylglyoxal with 1,2-diaminobenzene derivatives is investigated in detail, to find fluorogenic reagents for α-dicarbonyl compounds. Of the eight derivatives tested, 1,2- diamino-4,5-methylenedioxybenzene is shown to be the best reagent in terms of reactivity, selectivity and sensitivity. The reagent reacts with α-dicarbonyl compounds in very dilute hydrochloric acid and the products fluoresce intensely in neutral and alkaline media. The derivatives of seven α-dicarbonyl compounds are separated by reversed-phase h.p.l.c. The detection limits are in the range 65–280 fmol per 10-μl injection volume. The fluorescent products are characterized as the corresponding 2- and or 3-substituted 6,7-methylenedioxyquinoxalines.