S. Hara, Masaru Nakamura, F. Sakai
Jun 10, 1994
Citations
0
Influential Citations
8
Citations
Journal
Analytica Chimica Acta
Abstract
Abstract 2,2′-Dithiobis(1-amino-4,5-dimethoxybenzene) was synthesized as a highly sensitive and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with aromatic aldehydes in acidic media in the presence of tri- n -butylphosphine, sodium sulphite and disodium hydrogenphosphite. The reaction was complete within 60 min at 37°C. The fluorescent products from benzaldehyde and 4-hdyroxybenzaldehyde are shown to be 2-phenyl-5,6-dimethoxybenzothiazole and 2-(4-hydroxyphenyl)-5,6-di-reversed-phase chromatography and their detection limits (signal-to-noise ratio = 3) are 8–20 fmol on-column.