Y. Yasaka, M. Tanaka, T. Shono
1990
Citations
0
Influential Citations
66
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The use of 2-(2,3-naphthalimino)ethyl trifluoromethanesulphonate in the preparation of 2-(2,3-naphthalimino)ethyl ester derivatives of carboxylic acids for ultraviolet and fluorescent detection in high-performance liquid chromatography is described. The reagent is easily synthesized in two steps from 2,3-naphthalenedi-carboxylic anhydride and is stable at least for 6 months at room temperature. Reactions of carboxylate potassium salts (10 −5 M ) with a 10-fold equivalent excess amount of the reagent proceed to completion within 10 min in acetonitrile at room temperature in the presence of 18-crown-6 as a catalyst. The derivatization procedure with this reagent has been applied succesfully to the determination of some carboxylic acids in mouse brain. The detection limits (signal-to-noise ratio = 3) with ultraviolet and fluorescent detection are 100 fmol (at 259 nm) and 4 fmol (λ ex = 259 nm, λ em = 394 nm), respectively.