R. C. Lawrence
Mar 1, 1965
Citations
0
Influential Citations
26
Citations
Journal
Nature
Abstract
THE use of 2,4-dinitrophenyl hydrazine (2,4-DNP hydrazine) in acid solution as a reagent for the estimation of methyl ketones and other carbonyls is common despite the fact that the reaction is apparently non-quantitative1–3. Between 20 and 90 per cent recoveries of methyl ketones from aqueous solution have been reported4,5. Wong, Patton and Forss4 attributed the low recovery of 2-pentanone (25 per cent) from whole milk by steam distillation under reduced pressure to the non-completion of the reaction with the reagent. Haverkamp Begemann and de Jong5 concluded that the method of shaking had a marked influence on the yield of 2,4-DNP hydrazone, and by use of ‘Celite’ /2,4-DNP hydrazine column to ensure more intimate contact between the reagent and the carbonyl compound they achieved practically complete conversion into the 2,4-DNP hydrazone.