A. Velan, Srikumar Kotteazeth
Jan 1, 2018
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Quality indicators
Journal
International Journal of Clinical and Experimental Physiology
Abstract
Background and Aim: 28-homocastasterone (28-HC) is a keto oxysteroid comes under the brassinosteroid family phytohormone. 28-HC exhibited structural similarity with mammalian oxysteroid such as OH-cholesterol. Humans were exposed to 28-HC through diet and herbal-based medicine. Therefore, in the present study, we investigated the influence of 28-HC on hexokinase catalytic activity and mRNA expression in liver, kidney, and testicular tissues in normal and diabetic male rat. Methods: Induction of diabetes was achieved by single peritoneal injection of streptozotocin (60 mg/kg bwt) followed by 28-HC (333.33 μg/kg b.wt) which was fed orally for 15 days. At 16th day, tissues were removed followed by hexokinase activity, and mRNA expression was analyzed. In silico, docking study was performed to 28-HC against glucokinase and hexokinase proteins carried out using docking application AutoDock 4.0 suite docking simulations. Results: 28-HC-treated rat tissues showed significantly elevated hexokinase catalytic activity and mRNA expression (P < 0.05). On the other hand, in silico molecular, docking study was performed to recognize the binding affinity of 28-HC to glucokinase and hexokinase proteins. 28-HC was bound to the drug binding pocket of glucokinase and hexokinase. The glide energy score is − 6.23 and − 6.43 for glucokinase and hexokinase. Conclusion: 28-HC has high binding affinity for both glucokinase and hexokinase equally. Upregulation of hexokinase activity resulted in cellular glycolysis. Hence, it is suggested that 28-HC supplemented diets were suitable for higher energy-related work activities in human and animals.