H. Nohta, F. Sakai, M. Kai
Mar 21, 1994
Citations
0
Influential Citations
3
Citations
Journal
Analytica Chimica Acta
Abstract
Abstract 2-Amino-4,5-ethylenedioxyphenol was found to be useful as a precolumn fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with the aldehydes in two steps: first, heating (100°C) for 45 min in methanol solution in the presence of dicyclohexylcarbodiimide; secondly, standing for more than 1 min at room temperature after acidification. The fluorescent product from 4-hydroxybenzaldehyde is shown to be 2-(4-hydroxyphenyl)-5,6-ethylenedioxybenzoxazole. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase chromatography and their detection limits (S/N = 3) are 5–10 pmol on column.