A. Takadate, T. Masuda, Chikako Tajima
Oct 1, 1992
Citations
0
Influential Citations
31
Citations
Journal
Analytical Sciences
Abstract
3-Bromoacetyl-7-methoxycoumarin ( I ) was synthesized as a highly reactive fluorescent derivatization reagent for use in high-performance liquid chromatographic analysis. Compound I readily reacted with carboxylic acids in acetone at room temperature for 30 min in the presence of potassium bicarbonate and 18-crown-6 (Method A), or an anion-exchange resin (Method B) as catalysts to give the corresponding fluorescent carboxylic acid coumarinacyl esters ( II _ a - o). When lauric acid as a test sample was derivatized with I by use of Methods A and B, the detection limits were 0.4 and 0.5 pmol per injection ( S/N =3), respectively. These methods were applicable to the measurements of free fatty acids in human plasma (10–100 μl). Method B used a weakly basic anion-exchange resin instead of catalysts so in Method A, so it would be useful for the simplification of derivatization.