M. Yamaguchi, S. Hara, R. Matsunaga
1985
Citations
0
Influential Citations
77
Citations
Quality indicators
Journal
Journal of Chromatography A
Abstract
Abstract 3-Bromomethyl-6,7-dimethoxy-1-methyl-2(1 H )-quinoxalinone was found to be a selective and highly sensitive fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography. Its reactivity was investigated for various linear C 3 C 20 saturated fatty acids. The reagent reacts with the fatty acids in acetonitrile in the presence of 18-crown-6 and potassium carbonate to produce the corresponding fluorescent esters, which can be separated on a reversed-phase column, Radial-Pak C 18 cartridge, with gradient elution using 57–100% (v/v) aqueous methanol; the detection limits for the acids were 0.3–1 fmol for an injection volume of 5 μl. The reagent also reacts with unsaturated fatty, dicarboxylic, aromatic carboxylic and hydroxycarboxylic acids and acidic nucleotides to form fluorescent derivatives. α-Keto acids and α-amino acids do not give fluorescent derivatives under these conditions.