P. Chow, B. Weissmann
Oct 1, 1981
Citations
0
Influential Citations
22
Citations
Journal
Carbohydrate Research
Abstract
Abstract Condensation of dimeric 3,4,6-tri- O -acetyl-2-deoxy-2-nitroso-α- d -glucopyranosyl chloride with 4-methylumbelliferone gave crystalline 4-methylumbelliferyl 3,4,6-tri- O -acetyl-2-deoxy-2-oximino-α- d - arabino -hexopyranoside. Acetylation of this adduct, reduction of the resulting crude O -acetyloxime with borane in oxolane, and acetylation gave the 3,4,6-tri- O -acetyl derivative of 4-methylumbelliferyl 2-acetamido-2-deoxy-α- d -glucopyranoside ( 1 ). A new sensitive assay of N -acetyl-α- d -glucos-aminidase (E C 3.2.1.50) is made possible by fluorometric measurement of 4-methyl-umbelliferone liberated by enzymic hydrolysis of glycoside 1 . Such assays are illustrated by results obtained with enzyme preparations from pig liver and human-blood serum.