J. D. Hartog, J. H. Boom
Sep 2, 2010
Citations
0
Influential Citations
0
Citations
Journal
Recueil des Travaux Chimiques des Pays-Bas
Abstract
The synthesis of the crystalline and easily accessible phosphorylating agent 4-nitrophenyl 4-morpholinylphosphonochloridate is reported. Its application to the preparation of 5′-phosphorylated ribonucleosides (e.g. pA, ppA and pppA) as well as RNA fragments (e.g. pUpU and ppUpU) is demonstrated. Further, special attention is paid to the mild removal of the 4-nitrophenyl group from fully protected phosphotriester intermediates.