S. Kit, C. Beck, O. Graham
Jun 1, 1958
Citations
1
Influential Citations
41
Citations
Quality indicators
Journal
Cancer research
Abstract
Summary The effect of 5-bromodeoxyuridine and related compounds on the metabolism of lymphatic leukemias LL5147 and E9514A, lymphosarcoma 6C3-HED, rat thymus, and mouse spleen cells was studied both in vivo and in vitro . It was observed that 5-bromodeoxyuridine reduced the incorporation of formaldehyde-C 14 or formate-C 14 into DNA-thymine. The incorporation of the isotope into DNA-adenine or guanine was affected only at high concentrations of 5-bromodeoxyuridine. Several compounds related to bromodeoxyuridine had no significant effect. There was no inhibition of the labeling of free thymine compounds, of the hydroxymethylation reaction leading to serine synthesis, of the incorporation of the isotopic precursor into cell protein, or of cellular respiration or glycolysis. A slight but statistically questionable increase of the survival time of mice bearing ascites tumor E9514A was observed after the daily administration of 41.6 mg/kg body weight of 5-bromodeoxyuridine.