P. Brynes, P. Bevilacqua, A. Green
Sep 15, 1981
Citations
0
Influential Citations
28
Citations
Journal
Analytical biochemistry
Abstract
Abstract A new fluorogenic substrate capable of measuring the amidolytic activity of chymotrypsin and based upon the enzyme-catalyzed release of a highly fluorescent aromatic amine, 6-aminoquinoline, was prepared. The substrate, 6-( N -glutaryl- l -phenylalanylamido)quinoline, was found to have at pH 8.0 and 25°C K m = 1.77 m m and k cat = 1.4 × 10 −1 s −1 . The aminoquinoline is a unique leaving group in that its appearance can be measured fluorometrically at its excitation and emission maxima , while, under these conditions, fluorescence associated with unhydrolyzed substrate is negligible.