Tomohiko Yoshida, Y. Moriyama, H. Taniguchi
Jun 1, 1992
Citations
0
Influential Citations
5
Citations
Journal
Analytical Sciences
Abstract
6-Methoxy-2-methylsulfonylquinoline-4-carbonyl chloride was found to be a highly sensitive fluorescence derivatization reagent for both primary and secondary alcohols in high-performance liquid chromatography. Its reactivity was investigated for 1-propanol, benzyl alcohol, cyclohexanol, and 1-hexanol. The reagent reacted with the alcohols or phenol in benzene in the presence of pyridine to produce the corresponding fluorescent esters, which could be separated on a reversed-phase column, Cosmosil 5Cis-AR, with aqueous 70% (v/v) acetonitrile. The detection limits ( S/N =3) were 0.07 pmol for 1-propanol, 0.1 pmol for 1-hexanol and for benzyl alcohol, and 0.7 pmol for cyclohexanol for an injection volume of 10 µl.