C. Ong, Y. Ong, D. Vu
Nov 11, 2016
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Circulation
Abstract
Introduction: Sodium hydrosulfide (NaHS) has been shown to be a promising cardioprotective agent against ischemia-reperfusion injury, but its use as a component of cardioplegia in cardiopulmonary bypass (CPB) is untested. We investigated the potential cardioprotective effects of NaHS added to cardioplegia solution, in rats in cardiac arrest on CPB. Methods: Adult male Wistar rats (n=16) were placed on CPB and underwent 30 minutes of aortic cross-clamp (AoX)/ischemia, followed by 1 hour of reperfusion. Rats in the treatment group (n=8) received 40uM of NaHS in the cardioplegia solution, rats in the control group (n=8) did not. Hemodynamic parameters were measured intraoperatively and blood samples were collected at baseline, before and after AoX, and after weaning off CPB. Hearts were harvested at the end of the procedure. Results: The dP/dT max, indicative of systolic function, was better preserved in treatment groups post-CPB (32.7% decrease vs. 56.8% decrease from baseline) (p Conclusions: NaHS in cardioplegia solution significantly improves systolic function, diastolic function and ATP utilisation (which in turn results in improved hemodynamic parameters). It is superior to plain cardioplegia in reducing myocardial ischemia-reperfusion injury in the rat CPB and cardiac arrest model.