O. Suzuki, Y. Ishikawa, K. Miyazaki
Jun 3, 1986
Citations
0
Influential Citations
4
Citations
Journal
Biochimica et Biophysica Acta
Abstract
Abstract An acetylputrescine deacetylase was induced in Micrococcus luteus K-11, and was partially purified and characterized briefly. The enzyme was most active toward acetylputrescine, followed by N 8 -acetylspermidine and acetylcadaverine, but was inactive toward N 1 -acetylspermidine and N 1 -acetylspermine. The K m value for acetylputrescine was 0.321 mM. It was almost unaffected by -SH blocking agents but was inhibited by metal ions such as Cu 2+ and Ni 2+ . Its molecular weight estimated by Sephacryl S-200 column chromatography was 115000.