A. Hjerpe, C. A. Anotonopoulos, B. Classon
Jan 22, 1982
Citations
0
Influential Citations
21
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Two high-performance liquid chromatographic applications for the study of glucuronic and iduronic acid in glycosaminoglycans are presented. In the simplest form these uronic acids are separated on a reversed-phase column as their l- methylglycoside-6-methyl esters, i.e., the form in which they are recovered after methanolysis. The sensitivity for glucuronic acid is somewhat increased if the polysaccharide is deaminated prior to methanolysis, the subsequent separation being performed on a weak ion-exchange column. To allow this separation, however, an extra preparative step is necessary, consisting of alkaline hydrolysis of the ester bonds. Using these separations, the conditions for methanolysis were studied. Optimal release of uronic acids was achieved after 30–50 h of methanolysis using 1 M dry HCl in methanol at 100°C.