W. Osswald, W. Schütz, E. Elstner
Jun 1, 1989
Citations
0
Influential Citations
7
Citations
Journal
Journal of Plant Physiology
Abstract
Summary An in vitro system is described in which ethylene formation from the amino acid, 1-aminocyclopropane- 1-carboxylic acid (ACC), is catalyzed by horseradish peroxidase (HRP) in the presence of Mn 2+ and p-hydroxyacetophenone (pHAP) as cofactors. The monophenol can be substituted for by indole-3-acetic acid or indole-3-methanol. Oxidative ACC breakdown is accelerated at acidic pH whereas ethylene formation in the presence of IAA is stimulated by pHAP with a pH optimum at 7.8. ACC oxidation is inhibited by propylgallate and ascorbic acid by 100%, whereas OH scavengers such as mannitol or formate have no effect.