Chenjie Xu, Keming Xu, H. Gu
Feb 27, 2004
Citations
3
Influential Citations
392
Citations
Quality indicators
Journal
Journal of the American Chemical Society
Abstract
Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.