Minshen Chen, R. Whistler
Aug 1, 1975
Citations
0
Influential Citations
62
Citations
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Previous work from this laboratory has shown that 5-thio- d -glucose is a competitive inhibitor for active transport of d -glucose. The present work indicates that the thiosugar analog and its 1-phosphate can also interfere with d -glucose 6-P formation. 5-Thio- d -glucose serves as a substrate for yeast hexokinase with a Km of 4 m m , and V of 8.8 nmol/min/μg of protein. The analog competitively inhibits d -glucose phosphorylation with a Ki of 20 m m . 5-Thio- d -glucose 1-P can act as a substrate for rabbit skeletal muscle phosphoglucomutase with a Km of 60 μ m and V of 0.17 μmol/min/μg of protein. Thus, 5-thio- d -glucose 1-P behaves as a near metabolic analog of d -glucose 1-P. 5-Thio- d -glucose 1-P is a competitive inhibitor of d -glucose 1-P conversion to the 6-P with a Ki of 16.2 μ m . 5-Thio- d -glucose 6-P produced by phosphorylation of 5-thio- d -glucose and by conversion from 5-thio- d -glucose 1-P was identified by chromatographic mobility and by color reactions.