R. Melnick, J. D. de Sousa, J. Magiure
1975
Citations
0
Influential Citations
34
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Adenylyl imidodiphosphate (AMP-PNP), and analog of adenosine triphosphate (ATP), is a potent competitive inhibitor of mitochondrial ATPase activity. It inhibits both the soluble oligomycin-insensitive ATPase (Ki = 9.2 × 10−7 M) and the bound oligomycin-sensitive APTase (Ki = 1.3 × 10−6 M). ATPase activity of inside-out submitochondrial preparations are more sensitive to AMP-PNP in the presence of an uncoupler (Ki = 2.0 × 10−7 M). Mitochondrial ATP-dependent reactions (reversed electron transfer and potassium uptake) do not proceed if ATP is replaced with AMP-PNP; however, the analog does affect these systems. Oxidative phosphorylation of whole mitochondria and submitochondrial preparations were unaffected by AMP-PNP.