S. Clingenpeel, W. Keener, Caron R Keller
2005
Citations
0
Influential Citations
12
Citations
Journal
Journal of microbiological methods
Abstract
3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.