M. Wilchek, M. Gorecki
Dec 1, 1969
Citations
1
Influential Citations
83
Citations
Quality indicators
Journal
European journal of biochemistry
Abstract
5′-(4-Aminophenyl-phosphoryl)-uridine-2′(3′)-phosphate was synthesized and coupled to Sepharose by activation with cyanogen bromide. This conjugate was used as a specific adsorbent for purification of ribonuclease A by affinity chromatography. Totally reduced and oxidized RNase preparations which are enzymatically inactive were not adsorbed or retarded by the RNase-specific column, while S-protein is strongly retarded.