M. Inoguchi, H. Kamada, H. Harada
Oct 1, 1990
Citations
0
Influential Citations
4
Citations
Journal
Journal of Plant Physiology
Abstract
Summary Agropine synthesis in a Ti-transformed tobacco ( Nicotiana tabacum cv.Wisconsin 38) line was investigated in relation to tissue development.A teratoma was induced with Agrobacterium tumefaciens A66 and shoots were isolated in vitro .One of these showed agropine synthesis but without hormone autonomy.Southern blot analysis revealed that this line lacked the oncogenic genes of TL-DNA fragment but possessed a TR-DNA copy.Lack of oncogenic genes allowed plants to be regenerated repeatedly in vitro .Agropine content and synthetic activity of this transformant was consistently higher in callus tissues than in plant tissues.