Yujia Liu, Ayuki Kurita, Sayaka Nakashima
Aug 22, 2017
Citations
2
Influential Citations
16
Citations
Quality indicators
Journal
Bioscience, Biotechnology, and Biochemistry
Abstract
3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the major colonic microflora-produced catabolites of quercetin glycosides, such as quercetin 4′-glucoside derived from onion. Here, we investigated whether DOPAC modulates the aldehyde dehydrogenase (ALDH) activity and protects the cells from the acetaldehyde-induced cytotoxicity in vitro. DOPAC was shown to enhance not only the total ALDH activity, but also the gene expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. DOPAC simultaneously stimulated the nuclear translocation of NFE2-related factor 2 and aryl hydrocarbon receptor. The pretreatment of DOPAC completely protected the cells from the acetaldehyde-induced cytotoxicity. The present study suggested that DOPAC acts as a potential ALDH inducer to prevent the alcohol-induced abnormal reaction. 3,4-Dihydroxyphenylacetic acid, a colonic microflora-produced catabolite of quercetin glycosides, protects the cells from the acetaldehyde-induced cytotoxicity.