A. Gold, M. Osber
Feb 5, 1971
Citations
0
Influential Citations
21
Citations
Journal
Biochemical and biophysical research communications
Abstract
α-D-Glucopyranosyl fluoride (glucosyl fluoride) was found to be a substrate for sucrose phosphorylase from Pseudomonas saccharophila. At saturating Pi (0.030 M) the apparent Km is the same as that of sucrose, but the apparent Vmax is 2.7 times as great; therefore, glucosyl fluoride is the best glucose donor presently known for sucrose phosphorylase. The enzyme catalyzes hydrolysis of glucosyl fluoride at the same rate as sucrose and glucose-1-P.