D. J. Strydom
1996
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Influential Citations
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Journal
Techniques in Protein Chemistry
Abstract
Publisher Summary This chapter describes the amino acid analysis using carbamate reagents for precolumn derivatization. The introduction of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) as a derivatizing agent demonstrates the practical utility of a novel class of derivatizing agents that had been studied as activated carbamate reagents, but were not developed fully as amino acid analysis reagents. The AQC-derivatives are fluorescent and as a consequence have their greatest utility in sensitivity and selectivity of detection after chromatographic separation. They are very stable, in contrast to most other pre-column derivatives, because of their chemical structures of N -substituted ureas. The properties of AQC derivatives that lead to their decided advantage over include stability, sensitivity and selectivity, because of the fluorescent mode of detection. It can be used very accurately. The AQC derivatives provide optimal analyses of amino acids, with 4-phenylazoaniline-N-hydroxysuccinimidyl carbamate (PAPC) and anilino-N-hydroxysuccinimidyl carbamate (AC) providing solutions to at least two problem areas.