K. Biedermann, U. Montali, B. Martin
Jul 1, 1980
Citations
0
Influential Citations
18
Citations
Journal
Carlsberg Research Communications
Abstract
The amino acid sequence of yeast proteinase A inhibitor 3 (IA3) was deter mined to be Ac-Met-Asn-Thr-Asp-Gln-Gln-Lys-Val-Ser-Glu-Ile-Phe-Gln-Ser-Ser-Lys-Glu-Lys-Leu-Gln-Gly-Asp-Ala-Lys-Val-Val-Ser-Asp-Ala-Phe-Lys-Lys-Met-Ala-Ser-Gln-Asp-Lys-Asp-Gly-Lys-Thr-Thr-Asp-Ala-Asp-Glu-Ser-Glu-Lys-His-Asn-Tyr-Gln-Glu-Gln-Tyr-Asn-Lys-Leu-Lys-Gly-Ala-Gly-His-Lys-Lys-Glu.This structure was established by automated Edman degradation of peptides derived from cyanogen bromide fragmentation and enzymatic digestion with S. aureus V8 protease and chymotrypsin. High pressure liquid chromatography was used to separate the peptides from the different cleavage reactions. The cyanogen fragment residues 2–33 contained the inhibitory activity.