J. Kirschbaum, H. Brückner
Sep 1, 1996
Citations
0
Influential Citations
3
Citations
Journal
Chromatographia
Abstract
SummaryThe chromogenic reagent 4-phenylazobenzyloxycarbonyl chloride (PAZ-Cl) was used for the automated pre-column derivatization of amino acids (AAs) at ambient temperature followed by liquid chromatographic separation of the derivatives formed.Since in previous investigations it was realised that the selectivity of a C-18 stationary phase decreased after 40 injections of PAZ-AAs the suitability of 12 reversedphases distinguished by silica modification, carbon content, and particle characteristics was tested. Among these columns only a polymer-coated C-18 stationary phase furnished satisfactory repeatability of retention times for 250 analyses.