Su Chen, G. Kirschner, Emilio Benefenati
Jun 1, 1990
Citations
0
Influential Citations
10
Citations
Journal
Rapid Communications in Mass Spectrometry
Abstract
Negative-ion fast-atom bombardment mass spectrometry was evaluated as a means for quantitative analysis of individual molecular species of glycerophosphatidylserine (GPS). With 200 ng of 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (GPS di 16:0) as an internal standard, the calibration curve for 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine (GPS 18:0–18:1) was linear over a range of 50–1000 ng (signal-to-noise ratio >4), with a correlation coefficient of 0.998. When the curve was derived from a mixture of GPS molecular species, only a slight variation in the ion intensity ratio of GPS 18:0–18:1 to GPS di 16:0 occurred. The method provides the possibility of quantifying GPS 18:0–18:1 among GPS species in the low nanogram range.