F. Yufeng
2009
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Journal
China Modern Medicine
Abstract
Objective:The determination of the content of dihydralazine sulfate in compound reserpine tablets was estab-lished by eversed phase ion pair chromatograpy.Methods:A Agilent Eclipse XDB-C18 chromatographic column was used with acetonitrile-methanol-1.2% sodium heptanesulfonate(adjust pH to pH=2.5±0.1 by phosphoric acid)(20∶10∶70) as mobile phase at 28℃.The detecting wavelength was 310 nm.The flow rate was 1.0 ml/min.Results:The linear range was 0.078 5-0.784 8 μg(r=0.999 9).The average recoveries were 100.01%,99.82%,98.94% with corresponding RSD of 0.92%, 0.86%,1.07%.Conclusion:The method is simple, accurate and reproducible.It can be used in the quality control of this preparation.